منابع مشابه
Cholesterol Oxidase and Its Applications
Cholesterol oxidase (COX), a bi-functional FAD-containing microbial enzyme belongs to the family oxidoreductases. COX catalyses the oxidation of cholesterol into 4-cholesten-3-one. In recent time, cholesterol oxidase has received great attention due to its wider use in clinical (determination of serum cholesterol) laboratories practice and in the biocatalysis for the production of a number of s...
متن کاملCholesterol Oxidase: Source, Properties and Applications
Cholesterol oxidase (CHOx), a FAD-dependent enzyme of the oxido-reductase family catalyzes the oxidation of cholesterol to cholestenone. CHOx is produced by a large number of bacterial species, and the actinomycetes being most prolific group. Being an enzyme of great commercial value, CHOx has drawn significant attention due to its use in determination of cholesterol level in various clinical a...
متن کاملExtracellular Cholesterol Oxidase from Rhodococcus sp.: Isolation and Molecular Characterization
Background: Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical characterizations of produced CHO enzyme were described. Finally, CHO gene was cloned int...
متن کاملSTREPTOMYCES FRADIAE, A POTENT PRODUCER OF CHOLESTEROL OXIDASE
Thirteen species of Streptomycetes, Arthrobacters and Mycobacterium were studied for the determination of a potential cholesterol oxidase producer. Only Streptomyces fradiae and Streptomyces rishiriences were found to be capable of producing this enzyme. Streptomyces fradiae was selected for the production of cholesterol oxidase and cultured in different media to find optimum media and con...
متن کاملTracking cell cholesterol with cholesterol oxidase.
Sterols are ubiquitous and essential constituents of all eukaryotic cells, found either esterified and stored in droplet form (l), or unesterified and integrated into bilayers (2) . Sterols not only play a major structural role in membranes, but serve as precursors for steroid hormones and bile acids (3). The distribution of cholesterol within the cell and how it is apportioned properly are not...
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ژورنال
عنوان ژورنال: Advances in Microbiology
سال: 2012
ISSN: 2165-3402,2165-3410
DOI: 10.4236/aim.2012.22007